Abstract
Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.
Keywords
Fluorophore
Dichlorofluorescein
Fluorescence
Microcalcification
Chemistry
Biophysics
Medicine
Breast cancer
Biochemistry
Intracellular
Biology
Internal medicine
Cancer
Mammography
Optics
Subject Areas
Bone Metabolism and Diseases
·Molecular Biology ·Life Sciences
Connexins and lens biology
·Molecular Biology ·Life Sciences
S100 Proteins and Annexins
·Molecular Biology ·Life Sciences
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