Comparison of Mechanical Isolation Procedures of Stromal Vascular Fraction through Five Different Commercial Device

Abstract

Abstract Regenerative medicine has gained immense popularity in recent years due to its potential in treating various degenerative and inflammatory conditions, including osteoarthritis (OA). Adipose-derived mesenchymal stem cells (MSCs) and stromal vascular fraction (SVF) have been identified as promising cell sources in the field of regenerative medicine and orthopedics. Adipose tissue is an abundant and easily accessible source of MSCs, making it an attractive alternative to other sources, such as bone marrow. SVF is a heterogeneous population of cells that includes MSCs, endothelial cells, pericytes, and immune cells, among others. SVF can be obtained through enzymatic digestion of adipose tissue or by mechanical means, such as centrifugation or filtration. The advantage of mechanical methods is that they have fewer regulatory restrictions and are cost-effective for clinical use. Several commercial systems are available for the mechanical isolation of SVF, including Lipogems, Lipocell, Mystem, Lipocube Nano, and Lipocube Hybrid. However, there is limited data comparing the effectiveness of these systems in yielding viable cells with optimal regenerative potential. Therefore, this study aimed to compare these five commercial systems in terms of cell yield, cellular activity, and differentiation capacity.