Abstract
The covalent conjugates of cellulase from Aspergillus niger were prepared with various molar ratios by using dextran. The conjugate (nE/nD: 1/5) showed higher activity than purified enzyme at all temperatures after 1 h of incubation and its activity could also be measured at higher temperature. Also, this conjugate lost only 60% activity in 2 h at 70°C in comparison to the purified enzyme, which lost all its activity. In addition, conjugation protected cellulase against denaturation in the presence of sodium dodecylsulfate (residual activity of about 80%) and inactivation by air bubbles (residual activity of about 50% for 4 h).
Keywords
Chemistry
Aspergillus niger
Cellulase
Conjugate
Dextran
Covalent bond
Enzyme assay
Enzyme
Chromatography
Denaturation (fissile materials)
Specific activity
Nuclear chemistry
Biochemistry
Organic chemistry
Subject Areas
Biofuel production and bioconversion
·Biomedical Engineering ·Physical Sciences
Enzyme Catalysis and Immobilization
·Molecular Biology ·Life Sciences
Enzyme Production and Characterization
·Biotechnology ·Life Sciences
Citations by Year
2014: 1
2017: 1
2019: 1
2021: 2
OpenAlex SDG Match
SDGs auto-classified by OpenAlex (score ≥ 0.4 shown).
Clean water and sanitation 45%
Loading…
Loading…