Journal Article

·2023

Optimization of asymmetric bioreduction conditions of 1-(thiophen-2-yl)ethanone by Weissella cibaria N9 using a desirability function-embedded face-centered optimization model

Mohammed Bolubaid , Akın Özdemir , Enes Dertli̇ YTU , Mohammed Alamoudi , Osman Taylan , Derviş Karaboğa , Mustafa Tahsin Yılmaz YTU , Engin Şahin

Preparative Biochemistry & Biotechnology

Abstract

) on two response variables; (1) ee (%) and (2) cr (%) were tested. Next, desirability function-embedded face-centered optimization model revealed that a pH of 6.43, a temperature of 26.04 °C, an incubation period of 52.41 h, and an agitation speed of 150 rpm were the optimum levels and the estimated ee and cr responses were 99.31% and 98.16%, respectively. Importantly, the actual experimental ee and cr responses were similar to the estimated values indicating the capability of the offered desirability function-embedded face-centered optimization model when using the optimum cultural conditions.

Keywords

Chemistry Function (biology) Yield (engineering) Selectivity Biomass (ecology) Biocatalysis Organic chemistry Materials science Catalysis Composite material Reaction mechanism Biology

Subject Areas

Microbial Metabolic Engineering and Bioproduction ·Molecular Biology ·Life Sciences
Enzyme Catalysis and Immobilization ·Molecular Biology ·Life Sciences
Catalysis for Biomass Conversion ·Biomedical Engineering ·Physical Sciences