Peer-review

·2023 OPEN ACCESS

Peer Review #1 of "Prediction of HIV-1 protease resistance using genotypic, phenotypic, and molecular information with artificial neural networks (v0.2)"

Hüseyin Tunç YTU , Berna Doğan , Büşra Nur , Darendeli Kiraz YTU , Murat Sarı YTU , Serdar Durdağı , Seyfullah Kotil YTU , P Sharp , B Hahn , K Das ,

Abstract

Drug resistance is a primary barrier to effective treatments of HIV/AIDS.Calculating quantitative relations between genotype and phenotype observations for each inhibitor with cell-based assays requires time and money-consuming experiments.Machine learning models are good options for tackling these problems by generalizing the available data with suitable linear or nonlinear mappings.The main aim of this paper is to construct drug isolate fold (DIF) change-based artificial neural network (ANN) models for estimating the resistance potential of molecules inhibiting the HIV-1 protease (PR) enzyme.Throughout the study, seven of eight protease inhibitors (PIs) have been included in the training set and the remaining ones in the test set.We have obtained 11803 genotype-phenotype data points for eight PIs from Stanford HIV drug resistance database.Using the leave-one-out (LVO) procedure, eight ANN models have been produced to measure the learning capacity of models from the descriptors of the inhibitors.Mean R 2 value of eight ANN models for unseen inhibitors is 0.732, and the 95% confidence interval (CI) is [0.613,0.850].Predicting the fold change resistance for hundreds of isolates allowed a robust comparison of drug pairs.These eight models have predicted the drug resistance tendencies of each inhibitor pair with the mean 2D correlation coefficient of 0.933 and 95% CI [0.930,0.938].A classification problem has been created to predict the ordered relationship of the PIs, and the mean accuracy, sensitivity, specificity, and Matthews correlation coefficient (MCC) values are calculated as 0.954, 0.791, 0.791, and 0.688, respectively.Furthermore, we have created an external test dataset consisting of 51 unique known HIV-1 PR inhibitors

Keywords

Artificial neural network Genotype HIV-1 protease Phenotype Human immunodeficiency virus (HIV) Resistance (ecology) Protease Biology Computational biology Genetics Artificial intelligence Computer science Virology Gene Ecology Biochemistry

Subject Areas

HIV/AIDS drug development and treatment ·Infectious Diseases ·Health Sciences
HIV Research and Treatment ·Virology ·Life Sciences
Hepatitis C virus research ·Hepatology ·Health Sciences

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