Journal Article

·2013

Potentiometric glucose determination in human serum samples with glucose oxidase biosensor based on iodide electrode

Emine Karakuş YTU , Şule Pekyardımcı , E. Kiliç

Applied Biochemistry and Microbiology

Abstract

Glucose potentiometric biosensor was prepared by immobilizing glucose oxidase on iodide-selective electrode. The hydrogen peroxide formed after the oxidation of glucose catalysed by glucose oxidase (GOD) was oxidized by sodium molybdate (SMo) at iodide electrode in the presence ofdichlorometane. The glucose concentration was calculated from the decrease of iodide concentration determined by iodide-selective sensor. The sensitivity of glucose biosensor towards iodide ions and glucose was in the concentration ranges of 1.0 x 10(-1) - 1.0 x 10(-6) M and 1.0 x 10(-2) - 1.0 x 10(-4) M, respectively. The characterization of proposed glucose biosensor and glucose assay in human serum were also investigated.

Keywords

Glucose oxidase Biosensor Iodide Chemistry Potentiometric titration Hydrogen peroxide Inorganic chemistry Sodium iodide Electrode Molybdate Ion selective electrode Nuclear chemistry Chromatography Biochemistry Catalysis Selectivity Organic chemistry

Subject Areas

Electrochemical sensors and biosensors ·Electrical and Electronic Engineering ·Physical Sciences
Analytical Chemistry and Sensors ·Bioengineering ·Physical Sciences
Electrochemical Analysis and Applications ·Electrochemistry ·Physical Sciences

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