Abstract
Abstract Background Experimental studies performed with human olfactory nerve stem cells haveshown that these cells can ameliorate nerve cell regeneration. Developing a method of repairing nerve damage solely using stem cells without the need of any supporting material is important. Methods A multilayer cell mass was obtained from olfactory tissue-derived mesenchymal stem cells with high viability and proliferation capability using a protocol devoid of scaffolds or any other artificial supporting material. First, human olfactory tissue-derived mesenchymal stem cells were isolated, cultured, and characterized. Next, consecutive passages were conducted to obtain multilayer cell growth. The resulting cell mass could be suitable for tissue engineering models as well as nerve cell or tissue regeneration studies in the future. Results Viability and adhesive properties of the resulting cell mass were examined and found to be suitable for use in nerve tissue regeneration. Conclusion It is suggested that an in vitro -produced olfactory stem cell mass can be applied to a very small damaged region and could have a high potential for microenvironment formation.