Abstract
In this study, the urease-dextran non-covalent complexes in various molar ratios were synthesized and compared to the free enzyme in terms of pH, temperature, thermal and storage stabilities. Especially, the complex with a molar ratio of nU/nDA = 40/1 showed highest thermal stability and had ca. 1.4-fold at 25°C and 2.5-fold at 80°C higher activity than the free enzyme. The complex showed a high catalytic activity in organic solvent. In addition, the thermal and storage stabilities of urease were improved greatly as dextran complex, which has advantages for usage in practice.
Keywords
Chemistry
Dextran
Urease
Thermal stability
Enzyme
Catalysis
Molar ratio
Covalent bond
Immobilized enzyme
Chromatography
Organic chemistry
Subject Areas
Enzyme Catalysis and Immobilization
·Molecular Biology ·Life Sciences
Enzyme Production and Characterization
·Biotechnology ·Life Sciences
Biofuel production and bioconversion
·Biomedical Engineering ·Physical Sciences
Citations by Year
2019: 1
2020: 2
2021: 1
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